USP13 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

USP13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E8C10

Cat no : 66176-1-PBS

Synonyms

Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13

---

Validation Data Gallery

• 

  WB analysis using 66176-1-Ig (same clone as 66176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66176-1-Ig (same clone as 66176-1-PBS)

  WB result of USP13 antibody (66176-1-Ig, 1:600) with si-Control and si-USP13 transfected HeLa cells. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66176-1-Ig (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66176-1-Ig (USP13 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66176-1-Ig (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66176-1-Ig (same clone as 66176-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP13 antibody (66176-1-Ig, Clone: 6E8C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66176-1-PBS in a different storage buffer formulation.

---

Product Information

66176-1-PBS targets USP13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP13 fusion protein Ag10506
• Full Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Calculated Molecular Weight: 863 aa, 97 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC016146
• Gene Symbol: USP13
• Gene ID (NCBI): 8975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.