USP13 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 16840-1-AP

Synonyms

Ubiquitin-specific-processing protease 13, Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3

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Validation Data Gallery

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  WB analysis using 16840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP13 transfected HeLa cells.

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  WB analysis of rat testis using 16840-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16840-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 16840-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16840-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16840-1-AP

  WB result of USP13 antibody (16840-1-AP, 1:1000) with si-Control and si-USP13 transfected HeLa cells.

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  IP experiment of HeLa using 16840-1-AP

  IP result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16840-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16840-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using USP13 antibody (16840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, A375 cells, HEK-293 cells, rat testis tissue, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin cancer tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16840-1-AP targets USP13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP13 fusion protein Ag10527
• Full Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
• Calculated Molecular Weight: 863 aa, 97 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC016146
• Gene Symbol: USP13
• Gene ID (NCBI): 8975
• RRID: AB_2214569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Genes Dev; Chaperone-mediated autophagy degrades mutant p53.; Authors - Vakifahmetoglu-Norberg Helin H
human,mouse	WB,IHC,IF	EBioMedicine; The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.; Authors - Lian Li ; KD Validated
mouse,human	WB,IF	Transl Res; Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.; Authors - Xinxin Liao ; KO Validated
human	WB	J Cell Biol; Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.; Authors - Hong-Guang Xia
human	WB,IP	Oncogene; USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation; Authors - Cunying Ma ; KD Validated
mouse	IP	J Cell Physiol; IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.; Authors - Lian Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Francesca (Verified Customer) (05-22-2019)

Very good antibody for western blot. Antibody specificity validated by USP13 knock-down.

• Applications: Western Blot,
• Primary Antibody Dilution: 1: 2000 in 5% milk
• Cell Tissue Type: MeT-5A cells

USP13 Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16840-1-AP)