USP11 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

USP11 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6E7

Cat no : 67805-1-PBS

Synonyms

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis using 67805-1-Ig (same clone as 67805-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67805-1-Ig (same clone as 67805-1-PBS)

  WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67805-1-Ig (same clone as 67805-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67805-1-PBS in a different storage buffer formulation.

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Product Information

67805-1-PBS targets USP11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP11 fusion protein Ag19058
• Full Name: ubiquitin specific peptidase 11
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC000350
• Gene Symbol: USP11
• Gene ID (NCBI): 8237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.