USP11 Polyclonal antibody, PBS Only

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  WB analysis using 22340-1-AP (same clone as 22340-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

  WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 22340-1-AP (same clone as 22340-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 22340-1-AP (same clone as 22340-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

  IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 22340-1-AP (USP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 22340-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

22340-1-PBS targets USP11 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP11 fusion protein Ag17829
• Full Name: ubiquitin specific peptidase 11
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC000350
• Gene Symbol: USP11
• Gene ID (NCBI): 8237
• RRID: AB_2879079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51784
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.