USP10 Recombinant antibody

USP10 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240583G6

Cat no : 81435-2-RR

Synonyms

Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10

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Validation Data Gallery

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  WB analysis using 81435-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 81435-2-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 81435-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 81435-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81435-2-RR against Human USP10 were performed. The affinity constant is 3.99 nM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, NIH/3T3 cells, HEK-293 cells, A549 cells, LNCaP cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81435-2-RR is USP10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: USP10 fusion protein Ag7233
• Full Name: ubiquitin specific peptidase 10
• Calculated Molecular Weight: 87 kDa, 92 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC000263
• Gene Symbol: USP10
• Gene ID (NCBI): 9100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.