p115, USO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

p115, USO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 13509-1-AP

Synonyms

USO1, VDP, Transcytosis-associated protein, TAP, Protein USO1 homolog

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Validation Data Gallery

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  WB analysis of HeLa using 13509-1-AP

  WB result of p115, USO1 antibody (13509-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells.

• 

  WB analysis using 13509-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13509-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13509-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13509-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13509-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13509-1-AP (p115, USO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13509-1-AP

  IP result of anti-p115, USO1 (IP:13509-1-AP, 3ug; Detection:13509-1-AP 1:1500) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of human gliomas using 13509-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13509-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13509-1-AP (p115, USO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13509-1-AP (p115, USO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (13509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, mouse thymus tissue, HeLa cells, human brain tissue, SH-SY5Y cells, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 12 publications below

Product Information

13509-1-AP targets p115, USO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p115, USO1 fusion protein Ag4431
• Full Name: USO1 homolog, vesicle docking protein (yeast)
• Calculated Molecular Weight: 962 aa, 108 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC032654
• Gene Symbol: USO1
• Gene ID (NCBI): 8615
• RRID: AB_2257094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

Publications

Species	Application	Title
human	WB,IF	Mol Cell; An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.; Authors - Julian Nüchel
human	IF	Nat Commun; AmotL2 disrupts apical-basal cell polarity and promotes tumour invasion.; Authors - Mahdi Mojallal
human	WB,IF	J Cell Biol; Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.; Authors - Yijun Zhang
human	WB	J Cell Biol; PMID: 29133483; Authors - Junwan Fan
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed
mouse	IF	Cell Death Dis; Globozoospermia and lack of acrosome formation in GM130-deficient mice.; Authors - Feng Han

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