Harmonin Polyclonal antibody

Harmonin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11358-1-AP

Synonyms

USH1C, Renal carcinoma antigen NY-REN-3, Protein PDZ-73, Autoimmune enteropathy-related antigen AIE-75, Antigen NY-CO-38/NY-CO-37

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Validation Data Gallery

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  WB analysis using 11358-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11358-1-AP

  IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug.

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11358-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, mouse brain tissues, rat brain tissues
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: Human Colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11358-1-AP targets Harmonin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Harmonin fusion protein Ag1919
• Full Name: Usher syndrome 1C (autosomal recessive, severe)
• Calculated Molecular Weight: 552 aa, 62 kDa
• Observed Molecular Weight: 68-73 kDa
• GenBank Accession Number: BC016057
• Gene Symbol: USH1C/Harmonin
• Gene ID (NCBI): 10083
• RRID: AB_2214137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).

Publications

Species	Application	Title
mouse	WB,IF	Hum Mol Genet; Impact of the Usher syndrome on olfaction.; Authors - Fabian Jansen
human	IHC	Int J Mol Sci; A Liquid-Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients; Authors - Qi You