UROS Polyclonal antibody

UROS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14913-1-AP

Synonyms

Uroporphyrinogen-III synthase, Uroporphyrinogen-III cosynthase, uroporphyrinogen III synthase, UROIIIS, EC:4.2.1.75

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Validation Data Gallery

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  WB analysis using 14913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14913-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14913-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14913-1-AP

  IP result of anti-UROS (IP:14913-1-AP, 4ug; Detection:14913-1-AP 1:500) with K-562 cells lysate 4000ug.

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  IHC staining of human cervical cancer using 14913-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14913-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14913-1-AP

  Immunofluorescent analysis of MCF-7 cells, using UROS antibody 14913-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14913-1-AP targets UROS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UROS fusion protein Ag6711
• Full Name: uroporphyrinogen III synthase
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC002573
• Gene Symbol: UROS
• Gene ID (NCBI): 7390
• RRID: AB_2213824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.