URG4 Polyclonal antibody

• Featured Product
• KD/KO Validated

URG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11998-1-AP

Synonyms

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

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Validation Data Gallery

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  WB analysis of HEK-293 using 11998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11998-1-AP (URG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11998-1-AP

  WB result of URG4 antibody (11998-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-URG4 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 11998-1-AP

  IP result of anti-URG4 (IP:11998-1-AP, 4ug; Detection:11998-1-AP 1:300) with HEK-293 cells lysate 880ug.

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  IHC staining of human stomach cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11998-1-AP (URG4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 11998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11998-1-AP (URG4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 11998-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using URG4 antibody (11998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11998-1-AP targets URG4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: URG4 fusion protein Ag2630
• Full Name: up-regulated gene 4
• Calculated Molecular Weight: 922 aa, 104 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC018426
• Gene Symbol: URG4
• Gene ID (NCBI): 55665
• RRID: AB_1072845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

Publications

Species	Application	Title
human	WB	Bone; Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway; Authors - Yuanxi Leng
human	IHC,WB	J Orthop Surg Res; URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway.; Authors - Yayun Liu ; KD Validated