UQCRFS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UQCRFS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 18443-1-AP

Synonyms

Complex III subunit 5, Complex III subunit IX, Rieske iron sulfur protein, RIP1, RIS1, RISP, UQCR5, UQCRFS1

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Validation Data Gallery

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  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18443-1-AP (UQCRFS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 18443-1-AP

  IP result of anti-UQCRFS1 (IP:18443-1-AP, 4ug; Detection:18443-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18443-1-AP (UQCRFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IHC: See 2 publications below

Product Information

18443-1-AP targets UQCRFS1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UQCRFS1 fusion protein Ag13315
• Full Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010035
• Gene Symbol: UQCRFS1
• Gene ID (NCBI): 7386
• RRID: AB_10699875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
pig	WB	J Cachexia Sarcopenia Muscle; MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.; Authors - Ming Qi
human,mouse	WB	Cell Biosci; Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth; Authors - Hongting Zhao
mouse	WB	J Nanobiotechnology; Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation.; Authors - Li Xu
human	WB	Int J Mol Sci; Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells; Authors - Harpreet Kaur

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

ZEE (Verified Customer) (07-13-2020)

WE TRY THIS ANTIBODY WITH MOUSE HEART SAMPLES, AND GOT NICE RESULTS.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: ADULT MOUSE HEART