UQCRC2 Monoclonal antibody

UQCRC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1A5G11

Cat no : 67547-1-Ig

Synonyms

Complex III subunit 2, Core protein II, QCR2, UQCR2, UQCRC2

---

Validation Data Gallery

• 

  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, pig brain tissue, human placenta tissue, pig heart tissue, pig kidney, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67547-1-Ig targets UQCRC2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UQCRC2 fusion protein Ag6874
• Full Name: ubiquinol-cytochrome c reductase core protein II
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000484
• Gene Symbol: UQCRC2
• Gene ID (NCBI): 7385
• RRID: AB_2882763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li