UPF1 Recombinant antibody

UPF1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3O15

Cat no : 82791-1-RR

Synonyms

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

---

Validation Data Gallery

• 

  WB analysis using 82791-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 82791-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

• 

  Affinity and Kinetic Characterization of 82791-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82791-1-RR against Human UPF1 were performed. The affinity constant is 50.7 nM.

---

Tested Applications

• Positive WB detected in: rat testis tissue, Raji cells, SH-SY5Y cells, HEK-293 cells, HepG2 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:185-1:740

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82791-1-RR targets UPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UPF1 fusion protein Ag29057
• Full Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 123-130 kDa
• GenBank Accession Number: BC039817
• Gene Symbol: UPF1
• Gene ID (NCBI): 5976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.