UPF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UPF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 23379-1-AP

Synonyms

RENT1, Up-frameshift suppressor 1 homolog, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12

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Validation Data Gallery

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  WB analysis using 23379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 23379-1-AP

  IP result of anti-UPF1 (IP:23379-1-AP, 4ug; Detection:23379-1-AP 1:500) with Raji cells lysate 2000ug.

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  IF Staining of HepG2 using 23379-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UPF1 antibody (23379-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 23379-1-AP

  Immunofluorescent analysis of MCF-7 cells, using UPF1 antibody 23379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells, SH-SY5Y cells
• Positive IP detected in: Raji cells
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

23379-1-AP targets UPF1 in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPF1 fusion protein Ag16195
• Full Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 123-130 kDa
• GenBank Accession Number: BC039817
• Gene Symbol: UPF1
• Gene ID (NCBI): 5976
• RRID: AB_11232421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Breast Cancer Res; Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1.; Authors - Shuiyi Liu
human	WB	Bioengineered; The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.; Authors - Shizhen Sui
human	WB	J Exp Clin Cancer Res; PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.; Authors - Pingfu Hou
human	WB	Cell Death Dis; PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.; Authors - Rui Su