UNC45B Polyclonal antibody

UNC45B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 21640-1-AP

Synonyms

CMYA4, Protein unc 45 homolog B, SMUNC45, unc 45 homolog B (C. elegans), Unc 45B, UNC45, unc-45 myosin chaperone B, UNC45B

---

Validation Data Gallery

• 

  WB analysis using 21640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21640-1-AP (UNC45B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 21640-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21640-1-AP (UNC45B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21640-1-AP targets UNC45B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UNC45B fusion protein Ag16294
• Full Name: unc-45 homolog B (C. elegans)
• Calculated Molecular Weight: 931 aa, 104 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC101063
• Gene Symbol: UNC45B
• Gene ID (NCBI): 146862
• RRID: AB_10859787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Biol Cell; The myosin chaperone UNC-45 has an important role in maintaining the structure and function of muscle sarcomeres during adult aging; Authors - Courtney J Matheny

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

PK (Verified Customer) (08-14-2024)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: ac16

UNC45B Antibody Western Blot validation (1000 dilution) in ac16 (Cat no:21640-1-AP)