UNC45A Polyclonal antibody

• Featured Product
• KD/KO Validated

UNC45A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 19564-1-AP

Synonyms

Protein unc 45 homolog A, IRO039700, GCUNC-45, GCUNC45, GCUNC 45

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Validation Data Gallery

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  WB analysis of HeLa using 19564-1-AP

  WB result of UNC45A antibody (19564-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

• 

  WB analysis of K-562 using 19564-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19564-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19564-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19564-1-AP (UNC45A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 19564-1-AP

  IP result of anti-UNC45A (IP:19564-1-AP, 4ug; Detection:19564-1-AP 1:300) with K-562 cells lysate 3600ug.

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  IHC staining of human ovary cancer using 19564-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19564-1-AP (UNC45A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

19564-1-AP targets UNC45A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-45 homolog A (C. elegans)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_018671
• Gene Symbol: UNC45A
• Gene ID (NCBI): 55898
• RRID: AB_10644335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A.

Publications

Species	Application	Title
human	WB	FASEB J; A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.; Authors - Susana Lechuga ; KO Validated
rat	WB, IF	J Cell Sci; UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.; Authors - Juri Habicht
mouse	IHC,IF	Cells; UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.; Authors - Valentino Clemente

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kishor (Verified Customer) (10-17-2019)

This Ab works well for western blotting (1:1000) but I did not get satisfactory results for Immunofluorescence (1:200) in human liver cell lines.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: H69, HUCCT-1

UNC45A Antibody Western Blot,Immunofluorescence, validation (1:1000 and 1:200 dilution) in H69, HUCCT-1 (Cat no:19564-1-AP)