UNC45A Polyclonal antibody

• Featured Product
• KD/KO Validated

UNC45A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15479-1-AP

Synonyms

GC UNC45, GCUNC 45, GCUNC45, IRO039700, Protein unc 45 homolog A, SMAP 1, SMAP1, unc 45 homolog A (C. elegans), Unc 45A, UNC45A

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Validation Data Gallery

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  WB analysis of HeLa using 15479-1-AP

  WB result of UNC45A antibody (15479-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC45A transfected HeLa cells.

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  WB analysis using 15479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15479-1-AP (UNC45A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15479-1-AP

  IP result of anti-UNC45A (IP:15479-1-AP, 4ug; Detection:15479-1-AP 1:300) with HeLa cells lysate 1600 ug.

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  IP experiment of HeLa using 15479-1-AP

  IP result of anti-UNC45A (IP:15479-1-AP, 4ug; Detection:15479-1-AP 1:1000) with HeLa cells lysate 1400ug.

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  IHC staining of human cervix using 15479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervix using 15479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15479-1-AP (UNC45A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15479-1-AP

  Immunofluorescent analysis of HepG2 cells, using UNC45A antibody 15479-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervix tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

15479-1-AP targets UNC45A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UNC45A fusion protein Ag7790
• Full Name: unc-45 homolog A (C. elegans)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC006214
• Gene Symbol: UNC45A
• Gene ID (NCBI): 55898
• RRID: AB_2212736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.; Authors - Judd F Hultquist ; KO Validated
fish	ELISA	Food Res Int; Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).; Authors - Lihong Ge
human	IHC	Mol Cancer Res; PMID: 30322860; Authors - Ashley Mooneyham
human	WB,IF	Cancer Biol Ther; UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs.; Authors - Juri Habicht
human	WB,IP	J Biol Chem; Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors; Authors - Ya-Juan Wang
human,mouse	IF	Gastroenterology; Myosin Vb Traffics P-glycoprotein to the Apical Membrane of Intestinal Epithelial Cells; Authors - Sarah A Dooley