ULK1 Monoclonal antibody, PBS Only

ULK1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7G1

Cat no : 68445-1-PBS

Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Validation Data Gallery

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  WB analysis of PC-3 using 68445-1-Ig (same clone as 68445-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68445-1-Ig (same clone as 68445-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 68445-1-Ig (same clone as 68445-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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Product Information

68445-1-PBS targets ULK1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ULK1 fusion protein Ag22397
• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).