ULK1 Polyclonal antibody, PBS Only

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27352-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27352-1-AP (same clone as 27352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 27352-1-AP (same clone as 27352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 27352-1-AP (same clone as 27352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27352-1-AP (same clone as 27352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 27352-1-AP (same clone as 27352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 27352-1-AP (same clone as 27352-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using ULK1 antibody (27352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27352-1-PBS in a different storage buffer formulation.

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Product Information

27352-1-PBS targets ULK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ULK1 fusion protein Ag22397
• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318). ULK1 is a 113 kDa protein that it also can be detected a band of 150 kDa through the western blot (PMID: 32904616, PMID: 32899862, PMID: 37270528).