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ULBP2 Recombinant antibody, PBS Only

ULBP2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240413A6

Cat no : 83709-1-PBS

Synonyms

NKG2D ligand 2, N2DL-2, N2DL2, ALCAN-alpha, ALCAN alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ULBP2 Recombinant antibody (83709-1-RR)

    WB analysis using 83709-1-RR (same clone as 83709-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83709-1-RR (ULBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ULBP2 Recombinant antibody (83709-1-RR)

    IHC staining of human colon cancer using 83709-1-RR (same clone as 83709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ULBP2 Recombinant antibody (83709-1-RR)

    IHC staining of human colon cancer using 83709-1-RR (same clone as 83709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83709-1-RR

    Affinity and Kinetic Characterization of 83709-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83709-1-RR against Human ULBP2 were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 83709-1-PBS is ULBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name UL16 binding protein 2
Calculated Molecular Weight27 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberNM_025217.4
Gene Symbol ULBP2
Gene ID (NCBI) 80328
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.