Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68381-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 68381-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68381-1-Ig targets UGP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, Rat, Pig, Rabbit samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379) is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.
Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68381-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 68381-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
UGP2 Monoclonal antibody
Catalog Number
68381-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000
Applications
WB, IHC, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
