UGP2 Monoclonal antibody

UGP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, Rat, Pig, Rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11G6

Cat no : 68381-1-Ig

Synonyms

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

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Validation Data Gallery

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  WB analysis using 68381-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68381-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68381-1-Ig (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68381-1-Ig (UGP2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, pig liver tissue, LNCaP cells, rabbit liver tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68381-1-Ig targets UGP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: UGP2 fusion protein Ag33160
• Full Name: UDP-glucose pyrophosphorylase 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC002954
• Gene Symbol: UGP2
• Gene ID (NCBI): 7360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379) is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.