UGDH Polyclonal antibody

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  WB analysis using 13151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13151-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse liver using 13151-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 13151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using UGDH antibody (13151-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

UGDH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13151-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UDP-glucose 6-dehydrogenase, UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

13151-1-AP targets UGDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGDH fusion protein Ag3767
• Full Name: UDP-glucose dehydrogenase
• Calculated Molecular Weight: 494 aa, 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC022781
• Gene Symbol: UGDH
• Gene ID (NCBI): 7358
• RRID: AB_2214083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60701
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Publications

Species	Application	Title
human	WB	Nature; UDP-glucose accelerates SNAI1 mRNA decay and impairs lung cancer metastasis.; Authors - Xiongjun Wang ; KD Validated
human	WB, IHC	Arthritis Res Ther; UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis.; Authors - Yinxian Wen ; KD Validated
human	WB	PLoS One; Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.; Authors - Qi Ying
human	WB, IHC	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human,mouse	WB	Nat Commun; UDP-glucuronate metabolism controls RIPK1-driven liver damage in nonalcoholic steatohepatitis; Authors - Tao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain