UGCGL1 Polyclonal antibody

UGCGL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14170-1-AP

Synonyms

UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, UDP-glucose ceramide glucosyltransferase-like 1, UDP--Glc:glycoprotein glucosyltransferase, hUGT1

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Validation Data Gallery

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  WB analysis using 14170-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14170-1-AP (UGCGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14170-1-AP

  IP result of anti-UGCGL1 (IP:14170-1-AP, 5ug; Detection:14170-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 14170-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14170-1-AP (UGCGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14170-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14170-1-AP (UGCGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14170-1-AP targets UGCGL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UGCGL1 fusion protein Ag5369
• Full Name: UDP-glucose ceramide glucosyltransferase-like 1
• Calculated Molecular Weight: 1531 aa, 175 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC041098
• Gene Symbol: UGCGL1
• Gene ID (NCBI): 56886
• RRID: AB_1288973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1 at an apparent molecular mass of 170 kDa.

Publications

Species	Application	Title
rat	WB	J Cell Biol; Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins; Authors - Amber M Cheatham
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
rat	IF,WB	Toxicology; Tauroursodeoxycholic acid attenuates cisplatin-induced ototoxicity by inhibiting the accumulation and aggregation of unfolded or misfolded proteins in the endoplasmic reticulum.; Authors - Yingying Wen
human	WB	Glycobiology; Polypeptide N-acetylgalactosaminyltransferase 18 Retains in Endoplasmic Reticulum Depending on its Luminal Regions Interacting with ER resident UGGT1, PLOD3 and LPCAT1.; Authors - Wenjuan Jia
mouse	WB	Biochem Biophys Res Commun; UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner.; Authors - Jaeyong Cho