UFD1L Polyclonal antibody

• Featured Product
• KD/KO Validated

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 10615-1-AP

Synonyms

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1

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Validation Data Gallery

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  WB analysis of K-562 using 10615-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10615-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10615-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10615-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10615-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10615-1-AP

  IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.

• 

  IHC staining of human stomach using 10615-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10615-1-AP

  Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10615-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse thymus tissue, Raji cells, HepG2 cells, human testis tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10615-1-AP targets UFD1L in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UFD1L fusion protein Ag0954
• Full Name: ubiquitin fusion degradation 1 like (yeast)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC005087
• Gene Symbol: UFD1L
• Gene ID (NCBI): 7353
• RRID: AB_2213944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
human	WB	Nature; ESCRT-III controls nuclear envelope reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Elife; The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP; Authors - Maria Körner
mouse	WB	Sci Signal; TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing; Authors - Liangbin Lin
	WB	Plant J; The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.; Authors - Peifeng Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Leslie (Verified Customer) (10-08-2019)

Excellent antibody for Western blots

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Skeletal Muscle

UFD1L Antibody Western Blot, validation (1:1000 dilution) in Skeletal Muscle (Cat no:10615-1-AP)