UCP1 Recombinant antibody

• 

  WB analysis of mouse brown adipose using 83870-1-RR

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 83870-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 83870-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brown adipose using 83870-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brown adipose using 83870-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83870-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

UCP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83870-1-RR

Clone No.240782F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

---

Tested Applications

• Positive WB detected in: mouse brown adipose tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brown adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83870-1-RR targets UCP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UCP1 fusion protein Ag20388
• Full Name: uncoupling protein 1 (mitochondrial, proton carrier)
• Calculated Molecular Weight: 307 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC098168
• Gene Symbol: UCP1
• Gene ID (NCBI): 7350
• RRID: AB_3671456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P25874
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.