Urocortin Recombinant antibody

Urocortin Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241183B12

Cat no : 84380-1-RR

Synonyms

UCN, UROC, UI, 241183B12

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Validation Data Gallery

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  WB analysis using 84380-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84380-1-RR

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 84380-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 84380-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat kidney tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84380-1-RR targets Urocortin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: urocortin
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_003353
• Gene Symbol: UCN
• Gene ID (NCBI): 7349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).