UCHL5 Monoclonal antibody

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• KD/KO Validated

UCHL5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B8G11

Cat no : 68535-1-Ig

Synonyms

CGI 70, INO80R, Ubiquitin thioesterase L5, UCH L5, UCH37, UCHL5

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Validation Data Gallery

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  WB analysis using 68535-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68535-1-Ig (UCHL5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 68535-1-Ig

  WB result of UCHL5 antibody (68535-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UCHL5 transfected HCT 116 cells.

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Tested Applications

• Positive WB detected in: HCT 116 cells, LNCaP cells, MCF-7 cells, HeLa cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68535-1-Ig targets UCHL5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: ubiquitin carboxyl-terminal hydrolase L5
• Calculated Molecular Weight: 326 aa, 37 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC025369
• Gene Symbol: UCHL5
• Gene ID (NCBI): 51377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).