UCHL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

UCHL3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 12384-1-AP

Synonyms

Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, UCHL3, UCH-L3

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Validation Data Gallery

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  WB analysis using 12384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12384-1-AP (UCHL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 12384-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 12384-1-AP (UCHL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12384-1-AP

  IP result of anti-UCHL3 (IP:12384-1-AP, 4ug; Detection:12384-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IHC staining of human pancreas cancer using 12384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12384-1-AP (UCHL3 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, rat spleen tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

12384-1-AP targets UCHL3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCHL3 fusion protein Ag3053
• Full Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC018125
• Gene Symbol: UCHL3
• Gene ID (NCBI): 7347
• RRID: AB_2210915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer; Authors - Weina Zhang ; KD Validated
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nat Commun; Structural basis for specific inhibition of the deubiquitinase UCHL1; Authors - Christian Grethe ; KD Validated
human	WB	Genes Dev; A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination.; Authors - Kuntian Luo ; KO Validated
human	WB,IP	Oncogene; GSK3β and UCHL3 govern RIPK4 homeostasis via deubiquitination to enhance tumor metastasis in ovarian cancer; Authors - Wulin Shan
human	WB,IHC	Am J Cancer Res; UCHL3 promotes pancreatic cancer progression and chemo-resistance through FOXM1 stabilization.; Authors - Zhiwang Song ; KD Validated