UCH-L1/PGP9.5 Recombinant antibody, PBS Only

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  WB analysis using 84154-5-RR (same clone as 84154-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84154-5-RR (UCHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84154-5-RR (same clone as 84154-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-5-RR, Clone: 241107B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84154-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84154-5-RR against Human UCH-L1/PGP9.5 were performed. The affinity constant is 6.10 nM.

UCH-L1/PGP9.5 Uni-rAb^TM Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 84154-5-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84154-5-PBS targets UCH-L1/PGP9.5 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UCH-L1/PGP9.5 fusion protein Ag6490
• Full Name: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000332
• Gene Symbol: UCHL1
• Gene ID (NCBI): 7345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P09936
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).