UBXD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 14706-1-AP

Synonyms

UBXN6, UBXDC2, UBX domain-containing protein 6, UBX domain-containing protein 1

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Validation Data Gallery

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  WB analysis of PC-3 using 14706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14706-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBXD1 antibody (14706-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14706-1-AP (UBXD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, mouse brain tissue, human spleen tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

14706-1-AP targets UBXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBXD1 fusion protein Ag6394
• Full Name: UBX domain protein 6
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008288
• Gene Symbol: UBXD1
• Gene ID (NCBI): 80700
• RRID: AB_2210488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu ; KD Validated
human	IF	Acta Neuropathol Commun; Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation.; Authors - Feilin Liu
human	WB	J Immunol; UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.; Authors - Harshada Ketkar
human	IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli
human	WB	Sci Rep; Comprehensive analysis of endoplasmic reticulum stress related signature in head and neck squamous carcinoma; Authors - Yu Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tingting (Verified Customer) (01-16-2020)

Very clear bands

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Trophoblast cell, HEK293T