UBR5 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7A6

Cat no : 66937-1-PBS

Synonyms

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

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Validation Data Gallery

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  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66937-1-Ig (same clone as 66937-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66937-1-Ig (same clone as 66937-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66937-1-Ig (same clone as 66937-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66937-1-Ig (same clone as 66937-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66937-1-PBS targets UBR5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBR5 fusion protein Ag18753
• Full Name: ubiquitin protein ligase E3 component n-recognin 5
• Calculated Molecular Weight: 2799 aa, 309 kDa
• Observed Molecular Weight: 309 kDa
• GenBank Accession Number: BC137234
• Gene Symbol: UBR5
• Gene ID (NCBI): 51366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.