UBR4 Polyclonal antibody

UBR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15966-1-AP

Synonyms

RBAF600, p600, N-recognin-4, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR4

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Validation Data Gallery

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  WB analysis using 15966-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15966-1-AP

  IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug.

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  IHC staining of human pancreas cancer using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15966-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, mouse testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15966-1-AP targets UBR4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBR4 fusion protein Ag8740
• Full Name: ubiquitin protein ligase E3 component n-recognin 4
• Calculated Molecular Weight: 5183 aa, 570 kDa
• Observed Molecular Weight: 570 kDa, 270 kDa
• GenBank Accession Number: BC018694
• Gene Symbol: UBR4
• Gene ID (NCBI): 23352
• RRID: AB_2878202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.