UBR2 Polyclonal antibody

UBR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18853-1-AP

Synonyms

N recognin 2, KIAA0349, EC:2.3.2.27, E3 ubiquitin-protein ligase UBR2, C6orf133

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Validation Data Gallery

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  WB analysis of BxPC-3 using 18853-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18853-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18853-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18853-1-AP (UBR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18853-1-AP (UBR2 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of human ovary tumor using 18853-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18853-1-AP (UBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 18853-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using UBR2 antibody (18853-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 18853-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBR2 antibody 18853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse cerebellum tissue, mouse heart tissue
• Positive IHC detected in: human pancreas tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18853-1-AP targets UBR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBR2 fusion protein Ag13446
• Full Name: ubiquitin protein ligase E3 component n-recognin 2
• Calculated Molecular Weight: 439aa,50 kDa; 1755aa,201 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC064512
• Gene Symbol: UBR2
• Gene ID (NCBI): 23304
• RRID: AB_10638437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBR2(Ubiquitin-protein ligase E3-alpha-2) is also named as C6orf133, KIAA0349 and N-recognin-2. It is a component of the N-end rule pathway and recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It has four isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Interruption of the long non-coding RNA HOTAIR signaling axis ameliorates chemotherapy-induced cachexia in bladder cancer; Authors - Che-Yuan Hu