UBQLN3 Polyclonal antibody

UBQLN3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13568-1-AP

Synonyms

TUP 1, ubiquilin 3, Ubiquilin-3, UBQLN3

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Validation Data Gallery

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  WB analysis using 13568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13568-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13568-1-AP

  IP result of anti-UBQLN3 (IP:13568-1-AP, 4ug; Detection:13568-1-AP 1:800) with mouse testis tissue lysate 6000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13568-1-AP targets UBQLN3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBQLN3 fusion protein Ag4462
• Full Name: ubiquilin 3
• Calculated Molecular Weight: 655 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC036743
• Gene Symbol: UBQLN3
• Gene ID (NCBI): 50613
• RRID: AB_2241124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).

Publications

Species	Application	Title
human	WB,IHC	Fertil Steril; Potential biomarkers of nonobstructive azoospermia identified in microarray gene expression analysis.; Authors - Malcher Agnieszka A
mouse	WB	Mol Reprod Dev; Ubqln3, a testis-specific gene, is dispensable for embryonic development and spermatogenesis in mice.; Authors - N/A