UBP1 Polyclonal antibody

UBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13856-1-AP

Synonyms

LBP 1a, LBP 1B, LBP1, LBP1A, LBP1B, Transcription factor LBP 1, UBP1, Upstream binding protein 1

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Validation Data Gallery

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  WB analysis using 13856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13856-1-AP (UBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13856-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13856-1-AP (UBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13856-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13856-1-AP (UBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13856-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13856-1-AP (UBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, human kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13856-1-AP targets UBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBP1 fusion protein Ag4830
• Full Name: upstream binding protein 1 (LBP-1a)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC047235
• Gene Symbol: UBP1
• Gene ID (NCBI): 7342
• RRID: AB_2304131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.