UBLCP1 Polyclonal antibody

UBLCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12099-1-AP

Synonyms

UBLCP 1, Ubiquitin-like domain-containing CTD phosphatase 1, Nuclear proteasome inhibitor UBLCP1, EC:3.1.3.16, CPUB1

---

Validation Data Gallery

• 

  WB analysis of K-562 using 12099-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12099-1-AP (UBLCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12099-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12099-1-AP (UBLCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12099-1-AP

  IP result of anti-UBLCP1 (IP:12099-1-AP, 3ug; Detection:12099-1-AP 1:500) with K-562 cells lysate 1760ug.

• 

  IHC staining of human ovary tumor using 12099-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12099-1-AP (UBLCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12099-1-AP

  Immunofluorescent analysis of Hela cells, using UBLCP1 antibody 12099-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Tested Applications

• Positive WB detected in: K-562 cells, human brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12099-1-AP targets UBLCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBLCP1 fusion protein Ag2743
• Full Name: ubiquitin-like domain containing CTD phosphatase 1
• Calculated Molecular Weight: 318 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC013425
• Gene Symbol: UBLCP1
• Gene ID (NCBI): 134510
• RRID: AB_2256901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.