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UBE3A Monoclonal antibody

UBE3A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D6H9

Cat no : 60038-1-Ig

Synonyms

ANCR, AS, E6 AP, E6AP, E6AP ubiquitin protein ligase, EPVE6AP, HPVE6A, UBE3A, ubiquitin protein ligase E3A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using UBE3A Monoclonal antibody (60038-1-Ig)

    WB analysis of HeLa using 60038-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBE3A Monoclonal antibody (60038-1-Ig)

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UBE3A Monoclonal antibody (60038-1-Ig)

    IHC staining of human kidney using 60038-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60038-1-Ig targets UBE3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE3A fusion protein Ag0346
Full Name ubiquitin protein ligase E3A
Calculated Molecular Weight 852 aa, 98 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC002582
Gene Symbol UBE3A
Gene ID (NCBI) 7337
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.