UBE3A Polyclonal antibody

• Featured Product
• KD/KO Validated

UBE3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10344-1-AP

Synonyms

E6AP ubiquitin-protein ligase, E6AP ubiquitin protein ligase, E6AP, E6 AP, AS

---

Validation Data Gallery

• 

  WB analysis using 10344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10344-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10344-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10344-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10344-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10344-1-AP (UBE3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10344-1-AP

  IP result of anti-UBE3A (IP:10344-1-AP, 4ug; Detection:10344-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human thyroid cancer using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 10344-1-AP (UBE3A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 10344-1-AP (UBE3A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10344-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10344-1-AP (UBE3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10344-1-AP

  Immunofluorescent analysis of Hela cells, using UBE3A antibody 10344-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue, mouse lung tissue, Jurkat cells, NIH/3T3 cells, C6 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human thyroid cancer tissue, human kidney tissue, mouse lung tissue, rat liver tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 4 publications below

Product Information

10344-1-AP targets UBE3A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE3A fusion protein Ag0346
• Full Name: ubiquitin protein ligase E3A
• Calculated Molecular Weight: 852 aa, 98 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC002582
• Gene Symbol: UBE3A
• Gene ID (NCBI): 7337
• RRID: AB_2211801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479). It has 3 isoforms with MW of 97-101 kDa produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; Cas9 gene therapy for Angelman syndrome traps Ube3a-ATS long non-coding RNA.; Authors - Justin M Wolter
mouse	IF	J Mol Cell Biol; Landscape of genomic imprinting and its functions in the mouse mammary gland.; Authors - Haibo Xu
mouse,human	WB,IHC,IF,IP	Oncogene; Celastrol recruits UBE3A to recognize and degrade the DNA binding domain of steroid receptors; Authors - Qilong Tan ; KO Validated
human	WB,IF	Cell Death Discov; SNORD6 promotes cervical cancer progression by accelerating E6-mediated p53 degradation; Authors - Qianhui Li
human	WB,IF,IP	Int J Biol Sci; UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.; Authors - Zhikun Zheng ; KD Validated
mouse	WB	Mol Neurobiol; All-Trans Retinoic Acid Exerts Neuroprotective Effects in Amyotrophic Lateral Sclerosis-Like Tg (SOD1*G93A)1Gur Mice.; Authors - Yu Zhu