UBE2H Polyclonal antibody

UBE2H Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 15685-1-AP

Synonyms

UBC8, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme H, E2 20K

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Validation Data Gallery

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  WB analysis using 15685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15685-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15685-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15685-1-AP (UBE2H antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, mouse kidney tissue, HeLa cells, rat brain tissue, rat kidney tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15685-1-AP targets UBE2H in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2H fusion protein Ag8187
• Full Name: ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
• Calculated Molecular Weight: 183 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006277
• Gene Symbol: UBE2H
• Gene ID (NCBI): 7328
• RRID: AB_10858632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth; Authors - Nelson V Simwela
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

My attempt to use this antibody to detect UBE2H in HEK293T failed. The band intensity was very low, and there were many non-specific bands as well. I used bis-Tris gel and transferred the proteins to PVDF membrane. Primary antibody was incubated over night at 4'C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

UBE2H Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:15685-1-AP)