UBE2E3 Polyclonal antibody

UBE2E3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15488-1-AP

Synonyms

UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3

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Validation Data Gallery

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  WB analysis of Jurkat using 15488-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15488-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15488-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15488-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, mouse liver tissue, Y79 cells
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

15488-1-AP targets UBE2E3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2E3 fusion protein Ag7838
• Full Name: ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC003554
• Gene Symbol: UBE2E3
• Gene ID (NCBI): 10477
• RRID: AB_10896456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cells; Inhibition of miR-143-3p Restores Blood-Testis Barrier Function and Ameliorates Sertoli Cell Senescence; Authors - Ziyan Xiao
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe
mouse	WB	Int J Mol Sci; miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR; Authors - Jingxian Deng
monkey	WB	Sci Adv; TRIM37 is a primate-specific E3 ligase for Huntingtin and accounts for the striatal degeneration in Huntington's disease; Authors - Yiyang Qin