UBE2E2 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBE2E2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11844-1-AP

Synonyms

Ubiquitin-conjugating enzyme E2 E2, Ubiquitin protein ligase E2, Ubiquitin carrier protein E2, UBCH8, EC:2.3.2.23

---

Validation Data Gallery

• 

  WB analysis of human thyroid gland using 11844-1-AP

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11844-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 11844-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11844-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11844-1-AP (UBE2E2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11844-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBE2E2 antibody 11844-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: human thyroid gland tissue, human brain tissue, mouse embryo tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11844-1-AP targets UBE2E2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2E2 fusion protein Ag2509
• Full Name: ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
• Calculated Molecular Weight: 201 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC022332
• Gene Symbol: UBE2E2
• Gene ID (NCBI): 7325
• RRID: AB_2210274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).

Publications

Species	Application	Title
human	WB	Genes Dev; Sequential replication-coupled destruction at G1/S ensures genome stability.; Authors - Kate E Coleman ; KD Validated
human	WB,IHC	Cell Death Dis; UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer; Authors - Xiaoling Hong ; KD Validated
human	WB	Mol Cell Biol; Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.; Authors - Shibata Etsuko E
human	WB	Int J Mol Med; ISG15 inhibits cancer cell growth and promotes apoptosis.; Authors - Mei-Juan Zhou