Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, Hela cells, Jurkat cells, rat brain tissue, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11677-1-AP targets UBE2D1/2/3/4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2278 Product name: Recombinant human UBE2D3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC003395 Sequence: MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC003395 |
| Gene Symbol | UBE2D3 |
| Gene ID (NCBI) | 7323 |
| RRID | AB_2256691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61077 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBE2D1/2/3/4 antibody 11677-1-AP | Download protocol |
| WB protocol for UBE2D1/2/3/4 antibody 11677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-UBE2D3 antibody (Cat# 11677-1-AP) has 14 citations published in journals including Cell Research, Nature Communications, Molecular Cell, Cell Death Discovery, Arthritis & Rheumatology, Science Signaling, Oncotarget, and mSphere. Research fields span cancer biology (hepatocellular carcinoma, esophageal cancer, glioblastoma, breast cancer), ubiquitination, immune evasion, epigenetics, mTOR signaling, VEXAS syndrome, and virology (SARS-CoV-2). Applications include WB and IHC across human, mouse, hamster, and Drosophila species.
| Species | Application | Title |
|---|---|---|
Cell Res Tumor PD-L1 induces β2m ubiquitylation and degradation for cancer cell immune evasion. | ||
Nat Commun UBE2D3 facilitates NHEJ by orchestrating ATM signalling through multi-level control of RNF168
| ||
Nat Commun Clr4SUV39H1 ubiquitination and non-coding RNA mediate transcriptional silencing of heterochromatin via Swi6 phase separation | ||
Cell Death Discov Aberrant activation of a miR-101-UBE2D1 axis contributes to the advanced progression and chemotherapy sensitivity in human hepatocellular carcinoma | ||
