UBE2D1/2/3/4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15475-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15475-1-AP (same clone as 15475-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 15475-1-AP (same clone as 15475-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 15475-1-AP (same clone as 15475-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 15475-1-AP (UBE2D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15475-1-AP (same clone as 15475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15475-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15475-1-PBS in a different storage buffer formulation.

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Product Information

15475-1-PBS targets UBE2D1/2/3/4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2D1/2/3/4 fusion protein Ag7779
• Full Name: ubiquitin-conjugating enzyme E2D 4 (putative)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC004104
• Gene Symbol: UBE2D4
• Gene ID (NCBI): 51619
• RRID: AB_2210167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.