UBE2C Monoclonal antibody

• Featured Product
• KD/KO Validated

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

1C12H8

Cat no : 66087-1-Ig

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

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Validation Data Gallery

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  WB analysis using 66087-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66087-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66087-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, U2OS cells, A549 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 66087-1-Ig is UBE2C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBE2C fusion protein Ag19235
• Full Name: ubiquitin-conjugating enzyme E2C
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC016292
• Gene Symbol: UBE2C
• Gene ID (NCBI): 11065
• RRID: AB_11232220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	EMBO J; Shared and distinct mechanisms of UBA1 inactivation across different diseases; Authors - Jason C Collins
	WB	Elife; Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.; Authors - Andrew L Hong
human	IHC,IF	Cancer Lett; LINC01194 recruits NUMA1 to promote ubiquitination of RYR2 to enhance malignant progression in triple-negative breast cancer.; Authors - Duanyang Zhai
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu
human	WB,IHC	FEBS J; Silencing ubiquitin-conjugating enzyme 2C inhibits proliferation and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma.; Authors - Xianxing Wang ; KD Validated