UBE1 Monoclonal antibody, PBS Only

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7E8

Cat no : 67198-1-PBS

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

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Validation Data Gallery

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  WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67198-1-Ig (same clone as 67198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

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Product Information

67198-1-PBS targets UBE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBE1 fusion protein Ag8920
• Full Name: ubiquitin-like modifier activating enzyme 1
• Calculated Molecular Weight: 1058 aa, 118 kDa
• Observed Molecular Weight: 114-118 kDa
• GenBank Accession Number: BC013041
• Gene Symbol: UBA1
• Gene ID (NCBI): 7317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.