UBE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15912-1-AP

Synonyms

UBA1, GXP1, EC:6.2.1.45, AMCX1, A1ST

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 15912-1-AP

  WB result of UBE1 antibody (15912-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE1 transfected HEK-293 cells.

• 

  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15912-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15912-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15912-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 15912-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15912-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 15912-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 15912-1-AP (UBE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 15912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15912-1-AP (UBE1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, HEK-293 cells, mouse spleen tissue, rat spleen tissue, mouse colon tissue
• Positive IHC detected in: human colon cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below

Product Information

15912-1-AP targets UBE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE1 fusion protein Ag8703
• Full Name: ubiquitin-like modifier activating enzyme 1
• Calculated Molecular Weight: 1058 aa, 118 kDa
• Observed Molecular Weight: 114-118 kDa
• GenBank Accession Number: BC013041
• Gene Symbol: UBA1
• Gene ID (NCBI): 7317
• RRID: AB_2211462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE1(Ubiquitin-activating enzyme E1) is also named as A1S9T, UBE1 and belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation and initiates the activation and conjugation of ubiquitin-like proteins. Defects in UBE1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2). UBE1 has two isoforms with the molecular mass of 118 and 114 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IHC	ACS Nano; Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.; Authors - Yakun Wu
human		Cancer Biol Med; CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a.; Authors - Wenqi Du
human	WB	Am J Cancer Res; The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.; Authors - Zheng Xu
human	IHC	Front Oncol; Ubiquitin-Like Modifier Activating Enzyme 1 as a Novel Diagnostic and Prognostic Indicator That Correlates With Ferroptosis and the Malignant Phenotypes of Liver Cancer Cells.; Authors - Yiru Shan
human	WB	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang