UBC9 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBC9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 51018-2-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

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Validation Data Gallery

• 

  WB analysis of mouse thymus using 51018-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 51018-2-AP (UBC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart tissue using 51018-2-AP

  IP result of anti-UBE2I (51018-2-AP for IP and Detection) with mouse heart tissue lysate.

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Tested Applications

• Positive WB detected in: mouse thymus tissue
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

51018-2-AP targets UBC9 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC051289
• Gene Symbol: UBC9
• Gene ID (NCBI): 7329
• RRID: AB_2210591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Publications

Species	Application	Title
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human		Autophagy; PDPK1 regulates autophagosome biogenesis by binding to PIK3C3.; Authors - Boli Hu
mouse	WB	PLoS Genet; IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.; Authors - Bo Chen
pig	WB	Front Immunol; Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.; Authors - Xu Ye
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang ; KD Validated
human	WB	MBio; Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.; Authors - Sook-Young Sohn

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marie (Verified Customer) (03-05-2020)

Not working on rat cells/tissus. Working on Humain ( GFP-Ubc9 human overexpression)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: rat hippocampal culture

UBC9 Antibody Western Blot, validation (1/1000 dilution) in rat hippocampal culture (Cat no:51018-2-AP)