UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10224-1-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

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Validation Data Gallery

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  WB analysis of mouse spleen using 10224-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10224-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10224-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10224-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10224-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10224-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10224-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10224-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, HEK-293 cells, human testis tissue, Jurkat cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10224-1-AP targets UBC9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBC9 fusion protein Ag0252
• Full Name: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000427
• Gene Symbol: UBC9
• Gene ID (NCBI): 7329
• RRID: AB_2210593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).