UBC Polyclonal antibody

• Featured Product
• KD/KO Validated

UBC Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10457-1-AP

Synonyms

HMG20, Polyubiquitin C, UBC, ubiquitin C

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Validation Data Gallery

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  IHC staining of human lung cancer using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse liver tissue, human lung cancer tissue, human urothelial carcinoma tissue, mouse stomach tissue, rat liver tissue, rat pancreas tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

10457-1-AP targets UBC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBC fusion protein Ag0711
• Full Name: ubiquitin C
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: BC008955
• Gene Symbol: UBC
• Gene ID (NCBI): 7316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin (Ub) is a small, highly conserved eukaryotic protein that has an essential role in diverse cellular signaling pathways, including targeting proteins for proteasomal degradation. Cellular Ub is comprised of two distinct pools consisting of free Ub and Ub-substrate conjugates. UbC is a polyubiquitine gene that encodes Ub polyprotein. UbC, as stress-inducible polyubiquitin precursor proteins of around 9-11 monomers, is cleaved into monomeric ubiquitin by cellular deubiquitylating enzymes.

Publications

Species	Application	Title
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian ; KD Validated
		Chem Biol Interact; Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.; Authors - Zhan-Jun Ma
human	WB	Neuroscience; Maresin 1 Activates LGR6 to Alleviate Neuroinflammation via the CREB/JMJD3/IRF4 Pathway in a Rat Model of Subarachnoid Hemorrhage; Authors - Zhenyan Li