UBASH3A-Specific Polyclonal antibody

UBASH3A-Specific Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15823-1-AP

Synonyms

Cbl interacting protein 4, CLIP4, STS 2, STS2, T cell ubiquitin ligand, TULA, UBASH3A, UBASH3A-Specific

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Validation Data Gallery

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  WB analysis using 15823-1-AP

  mouse bone marrow tissue were subjected to SDS PAGE followed by western blot with 15823-1-AP (UBASH3A-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 15823-1-AP

  IP result of anti-UBASH3A-Specific (IP:15823-1-AP, 4ug; Detection:15823-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

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  IF Staining of THP-1 using 15823-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using UBASH3A-Specific antibody (15823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse bone marrow
• Positive IP detected in: mouse spleen tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

15823-1-AP targets UBASH3A-Specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin associated and SH3 domain containing, A
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: NM_018961
• Gene Symbol: UBASH3A
• Gene ID (NCBI): 53347
• RRID: AB_2272584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.

Publications

Species	Application	Title
human	WB	Diabetes; UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling.; Authors - Yan Ge
human	WB	Int J Mol Sci; UBASH3A Interacts with PTPN22 to Regulate IL2 Expression and Risk for Type 1 Diabetes; Authors - Jeremy R B Newman
human	WB	BMC Cancer; FLI1 induces erythroleukemia through opposing effects on UBASH3A and UBASH3B expression; Authors - Jie Wang
human	WB	Immunity; TCR signaling promotes formation of an STS1-Cbl-b complex with pH-sensitive phosphatase activity that suppresses T cell function in acidic environments; Authors - Yuan-Li Tsai
human	WB	J Immunol; UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes; Authors - Yan Ge
mouse	WB	Sci Signal; Inhibition of T cell activation and function by the adaptor protein CIN85.; Authors - Mei Suen Kong