UBAP1 Monoclonal antibody, PBS Only

UBAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6A2

Cat no : 66993-1-PBS

Synonyms

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

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Validation Data Gallery

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  WB analysis using 66993-1-Ig (same clone as 66993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66993-1-Ig (UBAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66993-1-Ig (same clone as 66993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66993-1-PBS in a different storage buffer formulation.

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Product Information

66993-1-PBS targets UBAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBAP1 fusion protein Ag17675
• Full Name: ubiquitin associated protein 1
• Calculated Molecular Weight: 502 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020950
• Gene Symbol: UBAP1
• Gene ID (NCBI): 51271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.